Melting Temperature Calculator

Melting temperature (Tm) is the temperature at which 50% of the DNA/RNA strands are in double-stranded form and 50% are in single-stranded form. It's a critical parameter for PCR primer design and hybridization experiments.

Several factors influence melting temperature, including sequence length, GC content, salt concentration, and DNA/RNA concentration. Our calculator accounts for these factors to provide accurate Tm predictions.

This tool is essential for PCR primer design, probe design, and hybridization experiments. Accurate Tm calculations are crucial for experimental success in molecular biology research.

While this tool focuses on melting temperature, you might also need to calculate GC content, primer annealing temperatures, or molecular weights. Check out our other biology tools for comprehensive sequence analysis capabilities.