Primer Design Tool

Primers are short DNA sequences that serve as starting points for DNA synthesis in PCR. They are essential components in molecular biology research, enabling the amplification of specific DNA regions through the polymerase chain reaction.

Simply enter your DNA sequence and adjust the parameters to match your requirements. The tool will automatically generate optimal primer pairs, considering factors like melting temperature, GC content, and potential secondary structures.

Melting temperature (Tm) determines primer binding efficiency, while GC content affects stability. The tool calculates these properties and ensures primers meet optimal specifications for successful PCR amplification.

For best results, aim for primers with balanced GC content (40-60%), appropriate length (18-25 bp), and similar melting temperatures. Avoid sequences with self-complementarity or repetitive regions that could form secondary structures.