Protein Isoelectric Point Calculator

The isoelectric point (pI) is the pH at which a protein has no net charge. This property is crucial for protein purification, electrophoresis, and understanding protein behavior in different pH environments.

Enter your amino acid sequence using the one-letter code. The tool will calculate the isoelectric point and provide a detailed amino acid composition analysis to help understand your protein's properties.

A protein's isoelectric point is determined by its amino acid composition, particularly the charged residues. The tool uses standard pKa values to calculate the net charge at different pH values and find the pI.

Knowledge of a protein's pI is essential for various applications, including isoelectric focusing, ion exchange chromatography, and predicting protein solubility and stability under different conditions.